General Stability | Organism |
---|---|
20% loss of activity after overnight dialysis against 10 mM potassium phosphate, pH 7.5. 50% loss of activity after overnight dialysis against 10 mM Tris-HCl, pH 7.5. Complete loss of activity after dialysis against water | Pseudomonas fluorescens |
freezing and thawing causes 20% inactivation | Pseudomonas fluorescens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Km for citrus pectin: 3.2 mg/ml | Pseudomonas fluorescens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
x * 32000, SDS-PAGE | Pseudomonas fluorescens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Pseudomonas fluorescens | constitutive enzyme, probably involved in the soft rot disease caused by Pseudomonas fluorescens | ? | - |
? | |
additional information | Pseudomonas fluorescens W51 | constitutive enzyme, probably involved in the soft rot disease caused by Pseudomonas fluorescens | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas fluorescens | - |
W51 | - |
Pseudomonas fluorescens W51 | - |
W51 | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas fluorescens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Pseudomonas fluorescens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | constitutive enzyme, probably involved in the soft rot disease caused by Pseudomonas fluorescens | Pseudomonas fluorescens | ? | - |
? | |
additional information | constitutive enzyme, probably involved in the soft rot disease caused by Pseudomonas fluorescens | Pseudomonas fluorescens W51 | ? | - |
? | |
pectin | - |
Pseudomonas fluorescens | ? | - |
? | |
pectin | - |
Pseudomonas fluorescens W51 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 32000, SDS-PAGE | Pseudomonas fluorescens |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | - |
room temperature, 5 min, 30% loss of activity | Pseudomonas fluorescens |
36 | - |
10 min, 30% loss of activity | Pseudomonas fluorescens |
42 | - |
5 min, 30% loss of activity | Pseudomonas fluorescens |
68 | - |
pH 7.8, 5 min, 97% loss of activity | Pseudomonas fluorescens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
8 | 8.5 | citrus pectin, Tris-HCl buffer | Pseudomonas fluorescens |